Effects of HRT on biological nitrogen removal in single-stage autotrophic process

Authors

  • Hong Liang
  • Shutong Liu
  • Xue Li
  • Xueying Sun
  • Dawen Gao

DOI:

https://doi.org/10.18063//AEB.2016.02.006.

Keywords:

hydraulic retention time, autotrophic biological nitrogen removal, deammonification, wastewater treatment

Abstract

An external circulation Sequencing Batch Reactor (ecSBR) was used to study the efficiency of nitrogen removal by autotrophic microbe. With gradually reducing the dissolved oxygen (DO) concentration from 1.2 mg/L to 0.04 mg/L, the single-stage autotrophic biological nitrogen removal (sABNR) process could be operated stably. After removing the aeration, the process could still stay sABNR stably, and the concentration of NH4+-N was 0.9 mg/L in effluent, the rate of nitrate (produced)/NH4+-N (removed) was in the range of 0.12–0.40. The results showed that the concentration of NH4+-N in effluent was 0.8, 0.8 and 9.9 mg/L with the hydraulic retention time (HRT) at 8 h, 6 h and 4 h respectively, the removal efficiency of ammonia were 98.2%, 98.1% and 73.6% respectively. The rate of nitrate (produced)/NH4+-N (consumed) was 0.05 at HRT 6 h, and the nitrogen loading rate (NLR) and nitrogen removal rate (NRR) were 169.7 and 129.7 g/m3/d, the removal efficiency of total nitrogen (TN) was 77.5%. In conclusion the optimal HRT was 6 h instead of 8 h or 4 h enough for ammonia removal without causing energy wastage.

 

References

Kartal B, Kuenen J G, and van Loosdrecht M C M, 2010, Sewage treatment with Anammox. Science, vol.328 (5979): 702–703. http://dx.doi.org/10.1126/science.1185941.

Zhang X J, Zhang H Z, Ye C M, et al. 2015, Effect of COD/N ratio on nitrogen removal and microbial communities of CANON process in membrane bioreactors. Bioresource Technology, vol.189: 302–308. http://dx.doi.org/10.1016/j.biortech.2015.04.006.

Vlaeminck S E, Geets J, Vervaeren H, et al. 2007, Reactivation of aerobic and anaerobic ammonium oxidizers in OLAND biomass after long-term storage. Applied Microbiology and Biotechnology, vol.74(6): 1376–1384. http://dx.doi.org/10.1007/s00253-006-0770-2.

Van Hulle S W H, Vandeweyer H J P, Meesschaert B D, et al. 2010, Engineering aspects and practical application of autotrophic nitrogen removal from nitrogen rich streams. Chemical Engineering Journal, vol.162(1): 1–20. http://dx.doi.org/10.1016/j.cej.2010.05.037.

Third K A, Paxman J, Schmid M, et al. 2005, Treatment of nitrogen-rich wastewater using partial nitrification and Anammox in the CANON process. Water Science and Technology, vol.52(4): 47–54.

Nielsen M, Bollmann A, Sliekers O, et al. 2005, Kinetics, diffusional limitation and microscale distribution of chemistry and organisms in a CANON reactor. Fems Micro-biology Ecology, vol.51(2): 247–256. http://dx.doi.org/10.1016/j.femsec.2004.09.003.

Yao Z B, Cai Q, Zhang D J, et al. 2013, The enhancement of completely autotrophic nitrogen removal over nitrite (CANON) by N2H4 addition. Bioresource Technology, vol.146: 591–596. http://dx.doi.org/10.1016/j.biortech.2013.07.121.

Liang Y H, Li D, Zhang X J, et al. 2014, Microbial characteristics and nitrogen removal of simultaneous partial nitrification, anammox and denitrification (SNAD) pro-cess treating low C/N ratio sewage. Bioresource Technology, vol.169: 103–109. http://dx.doi.org/10.1016/j.biortech.2014.06.064.

Innerebner G, Insam H, Franke-Whittle I H, et al. 2007, Identification of anammox bacteria in a full-scale deammonification plant making use of anaerobic ammonia oxidation. Systematic and Applied Microbiology, vol.30(5): 408–412. http://dx.doi.org/10.1016/j.syapm.2007.02.001.

Zhou J, Qin G X, Zhang J B, et al, 2014, Study on the mutual interactions between the parameters of a CANON system and its coping strategy when operating at room temperature (15 to 25 degrees C) using response surface methodology. Water Science and Technology, vol.69(9): 1805–1812. http://dx.doi.org/10.2166/wst.2014.062.

Du R, Peng Y Z, Cao S B, et al. 2015, Advanced nitrogen removal from wastewater by combining anammox with partial denitrification. Bioresource Technology, vol.179:497–504. http://dx.doi.org/10.1016/j.biortech.2014.12.043.

Varas R, Guzman-Fierro V, Giustinianovich E, et al. 2015, Startup and oxygen concentration effects in a continuous granular mixed flow autotrophic nitrogen removal reactor. Bioresource Technology, vol.190: 345–351. http://dx.doi.org/10.1016/j.biortech.2015.04.086.

Strous M, Van Gerven E, Kuenen J G, et al. 1997, Effects of aerobic and microaerobic conditions on anaerobic ammonium oxidizing (anammox) sludge. Applied and Environmental Microbiology, vol.63(6): 2446–2448.

Egli K, Fanger U, Alvarez P J, et al. 2001, Enrichment and characterization of an anammox bacterium from a rotating biological contactor treating ammonium-rich leachate. Archives of Microbiology, vol.175(3): 198–207.

Malovanyy A, Yang J J, Trela J, et al. 2015, Combination of upflow anaerobic sludge blanket (UASB) reactor and partial nitritation/anammox moving bed biofilm reactor (MBBR) for municipal wastewater treatment. Bioresource Technology, vol.180: 144–153. http://dx.doi.org/10.1016/j.biortech.2014.12.101.

Bagchi S R, Biswas and Nandy T, 2010, Alkalinity and dissolved oxygen as controlling parameters for ammonia removal through partial nitritation and ANAMMOX in a single-stage bioreactor. Journal of Industrial Microbiology & Biotechnology, vol.37(8): 871–876. http://dx.doi.org/10.1007/s10295-010-0744-3.

American Public Health Association (APHA), American Water Works Association (AWWA) and Water Environment Federation (WEF), 2005, Standard Methods for the Examination of Water and Wastewater, 20th edn. Washington, D.C.: American Public Health Association.

Fitzgerald C M, Comejo P, Oshlag J Z, et al. 2015, Ammonia-oxidizing microbial communities in reactors with efficient nitrification at low-dissolved oxygen. Water Research, vol.70: 38–51. http://dx.doi.org/10.1016/j.watres.2014.11.041.

Zhang X J, Li D, Liang Y H, et al. 2013, Autotrophic nitrogen removal from domestic sewage in MBR- CANON system and the biodiversity of functional microbes. Bioresource Technology, vol.150: 113–120. http://dx.doi.org/10.1016/j.biortech.2013.09.067.

Third K A, Sliekers O A, Kuenen J G, et al. 2001, The CANON system (Completely Autotrophic Nitrogen-re-moval Over Nitrite) under ammonium limitation: interaction and competition between three groups of bacteria. Systematic and Applied Microbiology, vol.24(4): 588–596. http://dx.doi.org/10.1078/0723-2020-00077.

Cai Y A, Li D, Liang Y H, et al. 2014, Autotrophic nitrogen removal process in a potable water treatment biofilter that simultaneously removes Mn and NH4+-N. Bioresource Technology, vol.172: 226–231. http://dx.doi.org/10.1016/j.biortech.2014.09.027.

Henze M, Harremoes P, Jansen J, et al. 2002, Wastewater Treatment: Biological and Chemical Processes, 3rd edn, Springer-Verlag, Berlin Heidelberg.

Kleerebezem R and R Mendez, 2002, Autotrophic denitrification for combined hydrogen sulfide removal from biogas and post-denitrification. Water Science and Technology, vol.45(10): 349–356.

Liu S, Yang F, Gong Z, et al. 2008, Application of anaerobic ammonium-oxidizing consortium to achieve completely autotrophic ammonium and sulfate removal. Bioresource Technology, vol.99(15): 6817–6825. http://dx.doi.org/10.1016/j.biortech.2008.01.054.

Vazquez-Padin J R, Pozo M J, Jarpa M, et al. 2009, Treatment of anaerobic sludge digester effluents by the CANON process in an air pulsing SBR. Journal of Hazardous Materials, vol.166(1): 336–341. http://dx.doi.org/10.1016/j.jhazmat.2008.11.055.

Vlaeminck S E, Terada A, Smets B F, et al. 2009, Nitrogen removal from digested black water by one-stage partial nitritation and anammox. Environmental Science & Technology, vol.43(13): 5035–5041. http://dx.doi.org/10.1021/es803284y.

Sliekers A O, Derwort N, Gomez J L, et al. 2002, Completely autotrophic nitrogen removal over nitrite in one single reactor. Water Resource, vol.36(10): 2475–2482. http://dx.doi.org/10.1016/S0043-1354(01)00476-6.

Ahn Y H and Choi H C, 2006, Autotrophic nitrogen re-moval from sludge digester liquids in upflow sludge bed reactor with external aeration. Process Biochemistry, vol.41(9): 1945–1950. http://dx.doi.org/10.1016/j.procbio.2006.04.006.

Cho S, Fujii N, Lee T, et al. 2011, Development of a simultaneous partial nitrification and anaerobic ammonia oxidation process in a single reactor. Bioresource Technology, vol.102(2): 652–659. http://dx.doi.org/10.1016/j.biortech.2010.08.031.

De Clippeleir H, Vlaeminck S E, De Wilde F, et al. 2013, One-stage partial nitritation/anammox at 15 °C on pre-treated sewage: feasibility demonstration at lab-scale. Applied Microbiology and Biotechnology, vol.97(23): 10199–10210. http://dx.doi.org/10.1007/s00253-013-4744-x.

Yang J J, Trela J, Zubrowska-Sudol M, et al. 2015, Intermittent aeration in one-stage partial nitritation/anammox process. Ecological Engineering, vol.75: 413–420. http://dx.doi.org/10.1016/j.ecoleng.2014.11.016.

.

Downloads

Published

2016-11-01

Issue

Section

RESEARCH ARTICLES