Draft genome sequence of Thauera sp. DTG from a denitrifying quinoline degrading microbial consortium

Authors

  • Guojun Wu
  • Menghui Zhang
  • Chaochun Wei
  • Yun Wang
  • Xiangyu Yao
  • Liping Zhao
  • Xiaojun Zhang

DOI:

https://doi.org/10.18063/AEB.2016.01.012

Keywords:

genome sequence, denitrifying, quinoline

Abstract

The draft genome sequence of Thauera sp. DTG was reconstructed from a metagenome of a denitrifying quinoline degrading microbial consortium. The organism is most closely related to Thauera aminoaromatic S2 and Thauera sp. MZ1T and is a facultative anaerobe. It is predicted to take the central role for quinoline denitrifying degradation.

References

Asakura S, Sawada S, Sugihara T, et al. 1997, QuinoLine-induced chromosome aberrations and sister chromatid exchanges in rat liver. Environmental and Molecular Mutagenesis, vol.30(4): 459–467. http://dx.doi.org/10.1002/(SICI)1098-2280(1997)30:4<459::AID-EM11>3.0.CO;2-C.

Zhang X, Yue S, Zhong H, et al. 2011, A diverse bacterial community in an anoxic quinoline-degrading bio-reactor determined by using pyrosequencing and clone library analysis. Applied Microbiology and Biotechnology, vol.91(2): 425–434.

http://dx.doi.org/10.1007/s00253-011-3296-1.

Mao Y, Zhang X, Xia X, et al. 2010, Versatile aromatic compound-degrading capacity and microdiversity of Thauera strains isolated from a coking wastewater treatment bioreactor. Journal of Industrial Microbiology & Biotechnology, vol.37(9): 927–934. http://dx.doi.org/10.1007/s10295-010-0740-7.

Liu B, Zhang F, Feng X, et al. 2006, Thauera and Azoarcus as functionally important genera in a denitrifying quinoline-removal bioreactor as revealed by microbial community structure comparison. FEMS Microbiology Ecology, vol.55(2): 274–286. http://dx.doi.org/10.1111/j.1574-6941.2005.00033.x.

Mechichi T, Stackebrandt E, Gad'on N, et al. 2002, Phylogenetic and metabolic diversity of bacteria degrading aromatic compounds under denitrifying conditions, and description of Thauera phenylacetica sp. nov., Thauera aminoaromatica sp. nov., and Azoarcus buck-elii sp. nov. Archives of Microbiology, vol.178(1): 26–35. http://dx.doi.org/10.1007/s00203-002-0422-6.

Allen M S, Welch K T, Prebyl B S, et al. 2004, Analysis and glycosyl composition of the exopolysaccharide isolated from the floc-forming wastewater bacterium Thau-era sp. MZ1T. Environmental Microbiology, vol.6(8): 780–790. http://dx.doi.org/10.1111/j.1462-2920.2004.00615.x.

Boll M and Fuchs G, 1995, Benzoyl-coenzyme A reductase (dearomatizing), a key enzyme of anaerobic aromatic metabolism. ATP dependence of the reaction, purification and some properties of the enzyme from Thauera aromatica strain K172. European Journal of Biochemistry, vol.234: 921–933. http://dx.doi.org/10.1111/j.1432-1033.1995.921_a.x.

Laempe D, Jahn M and Fuchs G, 1999, 6-Hydroxycy-clohex-1-ene-1-carbonyl-CoA dehydrogenase and 6-oxo-cyclohex-1-ene-1-carbonyl-CoA hydrolase, enzymes of the benzoyl-CoA pathway of anaerobic aromatic metabolism in the denitrifying bacterium Thauera aromatica. European Journal of Biochemistry, vol. 263(2): 420–429. http://dx.doi.org/10.1046/j.1432-1327.1999.00504.x.

Mao Y, Xia Y, Wang Z, et al. 2014, Reconstructing a Thauera genome from a hydrogenotrophic-denitrifying consortium using metagenomic sequence data. Applied Microbiology and Biotechnology, vol.98(15):6885–6895. http://dx.doi.org/10.1007/s00253-014-5756-x.

Gomez-Alvarez V, Teal T K and Schmidt T M, 2009, Systematic artifacts in metagenomes from complex microbial communities. The ISME Journal, vol.3: 1314–1317. http://dx.doi.org/10.1038/ismej.2009.72.

Schmieder R and Edwards R, 2011, Quality control and preprocessing of metagenomic datasets. Bioinformatics, vol.27(6): 863–864. http://dx.doi.org/10.1093/bioinformatics/btr026.

Dick G J, Andersson A F, Baker B J, et al. 2009, Community-wide analysis of microbial genome sequence signatures. Genome Biology, vol.10: R85. http://dx.doi.org/10.1186/gb-2009-10-8-r85.

Ultsch A and Mörchen F, 2005, ESOM-Maps: tools for clustering, visualization, and classification with Emergent SOM. Technical Report No. 46, Department of Mathematics and Computer Science, University of Mar-burg, Germany.

Rissman A I, Mau B, Biehl B S, et al. 2009, Reordering contigs of draft genomes using the Mauve Aligner. Bio-informatics, vol.25(16): 2071–2073. http://dx.doi.org/10.1093/bioinformatics/btp356.

Seemann T, 2014, Prokka: rapid prokaryotic genome annotation. Bioinformatics, vol.30(14): 2068–2069. http://dx.doi.org/10.1093/bioinformatics/btu153.

Downloads

Published

2015-04-01

Issue

Vol. 1 No. 1 (2016)

Section

Biodegradation and Bioremediation

License

Authors contributing to Applied Enviormental Biotechnology agree to publish their articles under the Creative Commons Attribution-Noncommercial 4.0 International License, allowing third parties to share their work (copy, distribute, transmit) and to adapt it, under the condition that the authors are given credit, that the work is not used for commercial purposes, and that in the event of reuse or distribution, the terms of this license are made clear.